Dr. Pingfang Yang
Room 302, Plant Biology building, WBGCAS
Wuchang Moshan, Wuhan 430074 China
yangpf@wbgcas.cn
ypf_bj@yahoo.com.cn
Education
1997 B.A., Northwest Sci-Tech University of Agriculture and Forestry, Shannxi, China, Economic Forestry
2002 M.S. Huazhong Agriculture University, Wuhan, China, Plant Embryology
2006 Ph.D. Institute of Botany, the Chinese Academy of Sciences, Beijing, plant proteomics
 
Professional experiences
07, 1997-02, 1998 A quality supervisor in a private Daily chemical company
03, 1998-08, 1999 An agrotechnician, Bureau of agriculture, Xiantao city, Hubei province, China
09, 1999-07, 2002 M.S. research. Trained in the field of Plant Embryology with micro-section and light microscope techniques. Using these techniques, figured out the reproduction in Eulaliopsis binata is apomixis
09, 2002-07, 2005 PhD research. Trained in plant proteomics. Using different proteomic techniques, studied the proteome profiles of rice under different stages or environments. Help to build up the whole proteome research system in this new lab
07, 2005-11, 2005 Research Assistant, Institute of Botany, the Chinese Academy of Sciences, Beijing. Except for the research work, also help to manage the lab and supervise PG students
11, 2005-02, 2007 Visiting Scholar, Hong Kong University of Science and Technology, Hong Kong. Get to be skilled with mammalian cell culture, RNAi techniques
3, 2007-12, 2009 Postdoc., DOE-Plant Research Laboratory, Michigan State University, East Lansing, MI. Involved in the study of plant peroxisome. Be skilled with organelle research techniques
12, 2009-now Research Scientists, PI, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuchang Moshan, Wuhan China
 
Honors
“Best Poster Award” in the 2nd International Symposium Frontier in Agriculture Proteome Research (ISFAPR) Nov 18-19th, 2010, Tsukuba, Japan
 
Research Interests
Molecular mechanism of Seed dormancy and germination
Plants reproduce their progenies mainly through the seeds. Seed germination, which is defined as the process that the mature seeds sense and transduct the suitable environmental factors like light, temperature and humidity, absorb the water quickly, activate a series of biochemical pathways and then emerge out of the capsule, is one of the important physiological processes in the life cycle of plants. This process is highly controlled by exogenous as well as endogenous factors. To understand how the seeds sense and transduct the exogenous signals, we will apply the proteomic strategy to identify the protein components that are involved in the signal transduction during the seed germination. Exploring the mechanism of seed germination will contribute a lot in solving the problems in both resource plant reproduction and agricultural production
Proteomics of plant sexual reproduction
After the vegetative growth, plants enter into the sexual growth period in which the flowers are developed. The development and maturation of microsporophyte and megasporophyte and their physiological status are critical for the success of the sexual reproduction. Stigma is the site where reception and recognition of pollens happen. The interaction and recognition between stigma and pollens are intermediated by a series of signal transduction. Aiming at exploring the mechanism of recognition, our group applies the proteomic strategy to study the proteome pattern of the mature stigma and its changes during this process. The identification of the functional important proteins will deepen our understanding of the sexual reproduction mechanism in the plant kingdom
 
Publications
First-author papers
1- Chi F*, Yang P*, Han F, Jing Y, Shen S. (2010) Proteomic Analysis of Rice Seedlings Infected by Sinorhizobium meliloti 1021. Proteomics, 10, 1861-1874. (co-first author)
2- Xiaoqin Wang, Pingfang Yang, et al., Exploring the mechanism of Physcomitrella patens desiccation tolerance through a proteomic strategy. Plant Physiology,149: 1739-1750 (co-first author)
3- Xiaoqin Wang*, Pingfang Yang*, et al. 2008, Proteomic analysis of the high-salinity stress in Physcomitrella patens. Planta, 228: 167-177 (co-first author)
4- Pingfang Yang, Xiaojuan Li, et al.2007, Proteomic Analysis of Rice Seeds during Germination. Proteomics 7: 3358-3368
5- Pingfang Yang, Hui Chen, et al. 2007, Proteomic Analysis of de-etiolated Rice Seedlings upon exposure to light. Proteomics, 7: 2459-2468
6- Pingfang Yang, Yu Liang, et al. 2006, Proteome study of rice uppermost internodes at the milk stage. Proteomics,6: 3330-3338
7- Ping-Fang Yang, Xiao-Juan Li, Yu-Xiang Jing, Yu Liang, Shi-Hua Shen and Ting-Yun Kuang. 2006, Proteomic analysis of the response of Liangyoupeijiu (a super high-yield hybrid rice) seedlings to cold stress. J Inte Plant Biol., 48(8): 945-951
8- Yang Pingfang, Shen Shihua and Kuang Tingyun. 2006, A Comparative Analysis of the Endosperm Proteins Separated by Two-dimensional Electrophoresis for two Cultivars of hybrid Rice (Oryza sativa L.). J Inte Plant Biol., 48(9): 1028-1033
 
Other papers
1- Yao Jia-lin, Yang Ping-fang, Hu Chun-gen, et al., 2004, Embryological Evidence for Apomixis in Eulaliopsis binata. Acta Botanica Sinica, 46(1): 86-92. (The first author is my supervisor in master degree study)
2- Qi Shuwei, Yang Pingfang, Shen Shihua, et al., 2004, Analysis of salt shock-induced proteome of Sinorhizobium fredii. Chinese Science Bulletin, 49:1828-1833
3- Yu Liang, Hui Chen, Ming-Juan Tang, Ping-Fang Yang, Shi-Hua Shen, 2007,  Responses of Jatropha curcas seedlings to cold stress: photosynthesis-related proteins and chlorophyll fluorescence characteristics.Physiologia Plantarum, 131(10): 508-517
4- Sigrun Reumann, Sheng Quan, Kyaw Aung, Pingfang Yang, et al., (2009) In-depth proteome analysis of Arabidopsis leaf peroxisomes combined with in vivo subcellular targeting verification uncovers low-abundance proteins with novel metabolic and regulatory functions. Plant physiology 150: 125-143
5- Wang Xiaoqin, Yang Pingfang, Zhang Xiaofeng, Xu Yinong, et al. (2009) Proteomic Analysis of Cold Acclimation in the Moss Physcomitrella patens. Proteomics, 9: 4529-4538